HOME » qPCR » qPCR Quality Control » gDNA contamination » Heat Labile dsDNase (HL-dsDNase)

Heat Labile dsDNase (HL-dsDNase)

1 2 3 4 5
(5/5) Based on 1 Rating
Units/Reactions:
280.00 EUR
842.00 EUR
1898.00 EUR

Information

Overview


Double-strand specific DNase (dsDNase) is a unique double-strand specific endonuclease. As it does not digest ssDNA, it can be used to remove dsDNA in the presence of single stranded DNA molecules, such as primers and probes. dsDNase is heat-labile, which makes it ideal for applications where the DNase have to be inactivated.

Heat inactivation


The heat labile dsDNase can be heat inactivated by treatment at 55°C for 15 min. If proceeding directly to PCR, 55°C for 5 min is sufficient. The enzyme requires 1 mM DTT and pH > 8 for inactivation.
Specifications

Unit definition


One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml of high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2.
Activity
HL-dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.

Storage


Minimum shelf life is 2 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.

Purity


HL-dsDNase is purified to apparent homogeneity.

Specific activity


Ca. 200 000 Kunitz Units/mg.

Specifications

Partno: 70800-201
Partno: 70800-202
Partno: 70800-203

Opinions

Add review

YUtC
Code from Captcha-image:

Rating (0)

Product documents

Document (0)

Other products of the same brand

  • ArcticZymes
    This enzyme will allow for removal of nucleic acids in a traditional protein ...
    from 130.00 EUR
  • ArcticZymes
    Shrimp Alkaline Phosphatase (SAP) – still the golden standard and the first h...
    from 104.00 EUR
  • ArcticZymes
    The Heat&Run gDNA removal kit removes contaminating gDNA from RNA prior to re...
    from 130.00 EUR
  • ArcticZymes
    The PCR Decontamination Kit removes contaminating DNA in PCR master mixes, wi...
    Art. no. 80400-100
    104.00 EUR