HOME » Assays and Oligos » 2-tailed assays for microRNA » Custom 2-tailed miRNA assays

Custom 2-tailed miRNA assays

1 2 3 4 5
(4.6/5) Based on 9 rating.
No price


Custom Two-tailed RT-qPCR microRNA assays

Our new TATAA miRNA 2-Tailed RT-qPCR assays show exceeding performance compared to other techniques as recently published in NAR (see Product Documents).

The challenge detecting small microRNAs is that two conventional PCR primers do not fit the target as their combined length is almost twice the length of the microRNA. Older techniques have solved this by extending the microRNA using eg., a hairpin primer, adding a poly A-tail, or adding a fragment by ligation. This, however, compromises the assay sensitivity and specificity, as only one of the PCR primers sense the actual microRNA sequence; the other senses the added extension. Further, these methods fail to detect microRNAs modified in the 3’-end as it interferes with the extension process.

TATAA miRNA 2-Tailed RT-qPCR assays offers a superior solution. Instead of using a single binding probe, Two-tailed PCR uses two hemiprobes, which bind to different stretches of the microRNA, that are connected by a folded tether. While each hemiprobe is too short to bind the microRNA, when both hemiprobes are complementary they bind cooperatively.


  • Binding is exceeding specific, as a mismatch is much more profound in a short hemiprobe
  • The cDNA formed can then be PCR amplified using two sequence specific primers
  • SYBR as well as hydrolysis probes can be used for detection
  • 1-tube color multiplexing is possible as well as high degree two-tube multiplexing of the RT followed by singleplex qPCR


How to order

To place an order, download the order form from Product Documents, fill it in and mail to: order@tataa.com

Two-tailed RT-qPCR assay is designed for microRNA target specified by client. The assay is validated on RT and qPCR level on synthetic microRNA.

The customer receives:

  • Assay sequences
  • Validation data report including standard curve and NTC control
  • Left-over primers (and probes for probe based assays) after finished validation

Shipping costs are not included, will be applied.



Partno: Custom design of new assays


Add review

Code from Captcha-image:

Rating (0)

You may also be interested in

  • TATAA Biocenter
    Robust and sensitive cDNA synthesis with user-defined RT priming
    from 149.00 EUR
  • Other products of the same brand

  • TATAA Biocenter
    Alu elements are short stretches of about 300 bp that are unique to the human...
    95.00 EUR
  • TATAA Biocenter
    Robust and sensitive cDNA synthesis with user-defined RT priming
    from 149.00 EUR
  • TATAA Biocenter
    Quantitative measurement of the biomining bacteria Acidithiobacillus caldus, ...
    Art. no. BQ-B01
    No price
  • TATAA Biocenter
    Detect and quantify the amount of human Y-chromosome
    Art. no. Y109P10
    150.00 EUR