HOME » qPCR » cDNA Synthesis » CelluLyser Lysis and cDNA Synthesis Kit - 100 reactions

CelluLyser Lysis and cDNA Synthesis Kit - 100 reactions

1 2 3 4 5
(5/5) Based on 2 rating.
Cellulyser kit:
749.00 EUR
300.00 EUR

Information

Rapid and sensitive lysis and reverse transcription of small cell samples in a single tube


The optimized CelluLyser™ buffer and powerful reverse transcription reagents maximize the yield of cDNA, making CelluLyser™ ideal for studies of small samples intended for real-time PCR. With the possibility to generate cDNA directly from cell lysate without isolating RNA, considerable amount of time is saved compared to standard RNA purification.

Description


The CelluLyser™ Lysis and cDNA Synthesis kit enables you to analyze small samples from 10 000 cells down to a single cell with minimal losses and hands-on time. The CelluLyser™ kit can be used with collected cells (FACS sorted, patch clamp collected cells etc.), as well as cultured cells (both adherent and in solution), and has successfully been tested on a number of different mammalian cell lines.

To avoid losses of material the entire procedure can be performed in a single tube without any sample transfer. Also, 100% of the total RNA content may be used from lysis to cDNA synthesis. The CelluLyser™ procedure is rapid and straightforward. Cells are lysed in only 10 minutes in the optimized CelluLyser™ buffer, and thereafter sensitive cDNA synthesis is performed directly on the cell lysate.

Contents


The reagents provided are sufficient either for 250 lysis reactions and 100 cDNA synthesis
reactions of 1 - 2 000 cells, or for 100 lysis reactions and 40 cDNA synthesis reactions
of 2 000 - 10 000 cells.
CelluLyserTM buffer: 1.25 ml (1X)

cDNA synthesis reagents (Roche Transcriptor First Strand cDNA Synthesis kit):
Transcriptor Reverse Transcriptase 50 µl (20 U/µl)
Transcriptor RT Reaction Buffer 1 ml (5x)
Protector RNase Inhibitor 100 µl (40 U/µl)
Deoxynucleotide Mix 200 µl (10 mM each)
Anchored-oligo(dT) Primer 200 µl (50 µM)
Random Hexamer Primer 200 µl (600 µM)
Water, PCR-grade 2x1ml

Specifications

Partno: H101
Partno: H102

Opinions

Add review

8YLT
Code from Captcha-image:

Rating (0)

Product documents

Document (1)

You may also be interested in

  • TATAA Biocenter
    The TATAA Probe GrandMaster® Mix is a 2x concentrated ready to use fastmix wh...
    from 480.00 EUR
  • TATAA Biocenter
    The TATAA SYBR® GrandMaster® Mix is a 2x concentrated ready to use fastmix wh...
    from 480.00 EUR
  • TATAA Biocenter
    Control for Primer Dimers in Probe based qPCR assays.
    Art. no. B102-6
    209.00 EUR
  • TATAA Biocenter
    Predominantly short fragments of DNA like free circulating DNA or in degraded...
    from 195.00 EUR
  • Other products of the same brand

  • TATAA Biocenter
    Detect and quantify the mount of human Y-chromosome The human Y-chromosome i...
    Art. no. Y109P10
    150.00 EUR
  • TATAA Biocenter
    The easy-to-use qPCR fastmix for all your gene expression and genotyping expe...
    from 251.00 EUR
    ( 310.00 EUR)
  • TATAA Biocenter
    The easy-to-use qPCR fastmix for all your gene expression experiments
    from 251.00 EUR
    ( 310.00 EUR)
  • TATAA Biocenter
    Assays designed for a microRNA target specified by the customer. To place an...
    Art. no. Custom design of new assays
    No price