The AccuBlue® Broad Range RNA Quantitation Kit is a fluorescence-based assay designed to quantify purified RNA samples in a fluorescent microplate reader or small fluorometer such as the Qubit® fluorometer (from Thermo Fisher Scientific). The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which corresponds to sample concentrations of 0.5 to 100 ng/uL. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR). Unlike absorbance-based measurements, RNA Broad Range Dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended).
The AccuBlue® Broad Range RNA Quantitation assay is designed for use with fluorescence microplate readers equipped with excitation and emission filters for detecting far-red fluorescence. It is also optimized for use in the Qubit® fluorometer, using the pre-programmed RNA Broad Range program.
Optimized for use on fluorescent microplate readers with excitation/emission of 630/660 nm
Optimized for use on Qubit® 3 fluorometers
Wide linear range of 5-1000 ng RNA (spanning those of both the Quant-iT™ RNA and Quant- iT™ RNA Broad Range kits from Thermo Fisher Scientific)
Highly selective for RNA over dsDNA, therefore no DNase step is necessary
RNA Broad Range Dye, 200X
RNA Broad Range Buffer
RNA Dilution Buffer (can use as Standard 1 on Qubit®)
RNA Broad Range Standard, 100 ng/uL (can use as Standard 2 on Qubit®)
Broad Range RNA Quantitation Kit designed for use with fluorescent microplate readers or with small fluorometers such as the Qubit® fluorometer (from Thermo Fisher Scientific). The assay is specific for RNA, and can quantify RNA samples in the range of 0.5-100 ng/uL.