Accel-NGS 2S PCR-Free Library Kit for Illumina
- Accel 2s PCR free kits:
Eliminate Sequencing Bias Introduced by Polymerase with PCR-Free Preps
The Accel-NGS 2S PCR-free DNA Library Kit for Illumina platforms enables preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed with two types of users in mind: those who have sufficient starting material to produce libraries free of bias from polymerase and those wishing to use a polymerase of choice for amplification. PCR-free libraries may be generated from as little as 100 ng of high quality genomic DNA or 10 ng of circulating cell-free DNA (cfDNA). PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.
- PCR-free libraries from inputs as low as 10 ng
- Excellent efficiency, data quality, and evenness of coverage
- No base composition bias from AT-rich or GC-rich genomes
- Optional library amplification with self-selected polymerase
The Accel-NGS 2S PCR-free kit is the right choice for users seeking unbiased coverage from relatively low input samples or who have a preferred polymerase for library amplification of very low input samples.
NGS Library Yields Without Bias
The Accel-NGS 2S PCR-free kit offers the same outstanding sequence coverage as the Accel-NGS 2S Plus DNA Library Kit, allowing significant cost and time savings for labs wishing to perform PCR-free preps or enabling a different library enrichment or amplification strategy.
Supported DNA Sequencing Applications and Sample Types
- Whole Genome Sequencing (WGS)
- Circulating Cell-free DNA (cfDNA)
- Targeted / Amplicon Sequencing
Accel-NGS 2S PCR-free utilizes Illumina-compatible adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.