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Accel-NGS 2S PCR-Free Library Kit for Illumina

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Accel 2s PCR free kits:
€ 665.00
€ 2470.00


Eliminate Sequencing Bias Introduced by Polymerase with PCR-Free Preps

The Accel-NGS 2S PCR-free DNA Library Kit for Illumina platforms enables preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed with two types of users in mind: those who have sufficient starting material to produce libraries free of bias from polymerase and those wishing to use a polymerase of choice for amplification. PCR-free libraries may be generated from as little as 100 ng of high quality genomic DNA or 10 ng of circulating cell-free DNA (cfDNA). PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.

  • PCR-free libraries from inputs as low as 10 ng
  • Excellent efficiency, data quality, and evenness of coverage
  • No base composition bias from AT-rich or GC-rich genomes
  • Optional library amplification with self-selected polymerase

The Accel-NGS 2S PCR-free kit is the right choice for users seeking unbiased coverage from relatively low input samples or who have a preferred polymerase for library amplification of very low input samples.

NGS Library Yields Without Bias

The Accel-NGS 2S PCR-free kit offers the same outstanding sequence coverage as the Accel-NGS 2S Plus DNA Library Kit, allowing significant cost and time savings for labs wishing to perform PCR-free preps or enabling a different library enrichment or amplification strategy.

Supported DNA Sequencing Applications and Sample Types

  • Whole Genome Sequencing (WGS)
  • Circulating Cell-free DNA (cfDNA)
  • Targeted / Amplicon Sequencing
  • Metagenomics
  • FFPE

Accel-NGS 2S PCR-free utilizes Illumina-compatible adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.


Swift Product Selection Guide


Partno: 20024
Partno: 20096


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