Accel-NGS 2S Hyb DNA Library Kit for Illumina
- Accel Hyb kits:
Minimize Duplicates, Maximize Sequencing Data
The Accel-NGS 2S Hyb DNA Library Kit designed for Illumina platforms produces 45% fewer duplicates at 1 ng to save you money on sequencing costs. A variety of indexing kits allow for compatiblity with multiple hybridization capture technologies: Agilent SureSelectXTand SureSelectXT2*, NimbleGen™ SeqCap™ EZ, and IDT® xGen® Lockdown® Probes.
- Validated as low as 1 ng human DNA
- Sequential repair steps for damaged samples
- Compatiblity with low input cfDNA and FFPE samples
- Increased library complexity
- Balanced coverage of AT-/GC-rich regions
The Accel-NGS 2S Hyb kit is the right choice for users working with samples of limiting quantity or quality who are looking to take advantage of the sequencing cost savings associated with hybridization capture.
Other Accel-NGS 2S Kits are available for users not performing hybridization capture. For users with at least 100 ng of consistently high quality DNA performing direct sequencing, please use the Accel-NGS 2S PCR-Free DNA Library Kit. For users with less than 100 ng of DNA or low quality DNA performing direct sequencing, please use the Accel-NGS 2S Plus DNA Library Kit.
High Complexity NGS Libraries for Hybridization Capture
The Accel-NGS 2S Hyb kit uses the same highly efficient library preparation technology as the Accel-NGS 2S Plus DNA Library Kit, expanding the sample types that can be analyzed to include low quality and low quantity samples. The high complexity libraries constructed by the Accel-NGS 2S Hyb kit enable researchers to extract meaningful targeted sequencing data from samples that would fail with less efficient library preparations.
Supported DNA Sequencing Applications and Sample Types
- Whole exome sequencing (WES)
- Specific gene panel targeted sequencing
- Low input FFPE samples
- Low input circulating, cell-free DNA (cfDNA)
Accel-NGS 2S Hyb utilizes Illumina-compatible adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.