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Vendor: Norgen Biotek
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Purification and enrichment of intact urinary exosomes for functional studies
Bind and elute all RNA irrespective of size or GC content, without bias
Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA?
Versatile urine input volume (2 mL to 10 mL)
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes are required
No precipitation reagents nor overnight incubation required
Compatible with urine from any species
Pure exosomes are purified and free-from any other RNA-binding proteins
Urine Exosome Purification and RNA Isolation Midi Kit
Norgen’s Exosome Purification and RNA Isolation Midi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes ranging from 2 mL to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
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