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The QuantSeq FWD Kit is an all-in-one library preparation protocol designed to generate Illumina compatible libraries of sequences close to the 3’ end of polyadenylated RNA.
The kit is ideal for gene expression counting as NGS reads are generated towards the poly(A) tail. To get the exact 3’ transcript end longer reads are necessary.
QuantSeq FWD Kits are delivered with several indexing options:
Unique Dual Indexing with the Lexogen UDI 12 nt Sets (Cat. 113.96, 129-131.96, 114.96, 115.384) with up to 384 Unique Dual Indices.
Single Indexing with the Lexogen i7 6 nt Index Sets (Cat. No. 015.24/96/2×96)
Combinatorial Dual Indexing with the Lexogen i7 6 nt Index Set and i5 6 nt Dual Indexing Add-on Kit (015.384)
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